| 1. | The optimized condition is : urease concentration 8 mg l " 1 , ph value of subphase 5 . 5 , association time of ureaes with amphiphiles 2 h , the temperature of subphase 10 ? , sio2 / si substrate with hydrophobic surface 脲酶浓度8mgl ~ ( - 1 ) ,亚相ph值5 . 5 ,酶与表面单分子膜的缔合时间2h ,亚相的温度10 ,基片表面为憎水性。 |